Characterization of recombinant staphylococcal enterotoxin H (rSEH) and the anti-rSEH IgGs. (A) Verification of His-tagged rSEH by western blot; (B) confirmation of prepared rSEH by 12% SDS-PAGE; (C) assessment of the reactivity and specificity of the anti-rSEH IgGs by western blot.Lane M, protein molecular mass marker; Lane 1, His 6-rSEH fusion protein purified by nickel affinity ... More @Wikipedia
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