1. ORG (abbr. for ORiGin) is an assembly directive and is not an instruction. It defines where the machine code (translated assembly program) is to place in memory. As for ORG 100H this deals with 80x86 COM program format (COMMAND) which consists of only one segment with a maximum of 64k bytes. More @Wikipedia
Hover over any link to get a description of the article. Please note that search keywords are sometimes hidden within the full article and don't appear in the description or title.